AbhayJere.com
India is World's New BioSciences Hub
Home
President's Desk
Bio-Pharmaceuticals
Bioinformatic Tools
Science Blogs
Free Online Books *New
Competitive Exams* New
Columns (Updated)
Databases
Interviews & Profiles
JOB Links
Journals Impact Factor
Science News
Online Protocols
Research Institutions **
Scholarships* New
Science Search Engines
Tutorials (Updated)
Science Movies
Universities-USA & India
Contact Us
Introduction
1.1 The Bright Field Microscope
1.2 Use of Oil Immersion (100x)
1.3 Measurements: Ocular and Stage Micrometers
1.4 Measuring Depth
1.5 Measuring Area
1.6 Measuring Volume
1.7 Measurement of Cell Organelles
1.8 Use of Darkfield Illumination
1.9 The Phase Contrast Microscope
1.10 The Inverted Phase Microscope
Electron Microscopy
Introduction
Electron Microscopy of the Cytoskeleton of Cultured Cells
Protocols
Routine TEM Staining Protocol for Tissues
Routine TEM Staining Protocol for Cells
Routine TEM Processing and Staining Protocol for Paraffin Sections Mounted on Slides
L.R.White Embedding Protocol for TEM
Toluidine Blue Staining for TEM Thick (Semithin) Sections
EM Immunogold Labeling Protocol - Pre-embedding Method
EM Immunogold Labeling Protocol - Post-embedding Method-1
EM Immunogold Labeling Protocol - Post-embedding Method-2
EM Negative Staining Protocol
Confocal Microscopy /
Fluorescence Microscopy
Introduction
Protocol
Immunofluorescence / confocal microscopy
What is a Fluorescence Microscope?
Basic Requirements of Fluorescence Microscope Optics
The Dichroic Mirror
Excitation and Emission Filters
The Filter Cube
Problem: The Need for Quick Wavelength Changes
Fluorochrome Table
,
Protocols
Fixation
Permeabilization and Blocking
Staining
,
Mounting
Immunofluorescence-Cell Surface Staining
Immunofluorescence Protocol for Cultured Cells
Immunofluorescence Protocol for Cultured Cells-2
Making Fluorescence Mounting Medium (ANTIFADE)
Immunofluorescence - Whole Mount
Whole Mount Immunochemical Fluorescence Confocal Microscopy
Yeast IF with Methanol/Acetone Dehydration
Yeast IF without Dehydration
Immunofluorescence of C. elegans Embryos
(Aroian Lab)
Indirect Immunofluorescence
Two-Colour Immunofluorescence
Three-Colour Immunofluorescence
Double Immunofluorescence Staining Protocol
Double Immunofluorescence Protocol
All rights reserved (Copyright 2006)