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Sequence Alignment Tools Phylogenetic analysis Tools Phylogenetic programs | List of phylogenetic packages and free servers (PHYLIP pages) | PHYLIP | Server for phylogenetic analysis using the PHYLIP package | BIONJ | Server for Neighbour Joining phylogenetic analysis | TREE PUZZLE | Package for phylogenetic analysis by Maximum Likelihood | PHYML | Server for Maximum Llikehood phylogenetic analysis | SAPS | Statistical analysis of protein sequences at EMBnet-CH |
Primer and PCR Design Tools FastPCR | Excellent free downloadable PCR primer design software and can design primers for standard and long PCR, inverse PCR, degenerate PCR directly on amino acid sequence, multiplex PCR | Electronic PCR | Electronic PCR (e-PCR) is computational procedure that is used to identify sequence tagged sites(STSs), within DNA sequences. | Primer 3 | Selects PCR primers from nucleotide sequences | PrimerQuest | Tool for the design of PCR primers, probes, and sequencing primers | Primer Design Assistant (PDA) | A web-based primer design tool | MethPrimer | Design Primers for Methylation PCRs. The only free public methylation primer program available. | AutoPrime | Freely accessible web-based primer design software which designs primers of high quality for Real-Time-Quantitative PCR |
Restriction Enzyme Mapping Tools WebCutter | Web-based tool for restriction mapping of DNA sequences | NEBcutter V.2.0 | For all Type II and commercially available Type III restriction enzymes that cut the sequence just once |
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